Last data update: 24 January 2024 16:39 CET
Host name: Escherichia coli K12 W3110 Sparrowhawk (LMBP 13419)
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| Species: | Escherichia coli |
| Group: | K12 |
| Strain designation: | W3110 Sparrowhawk |
| Accession number: | LMBP 13419 |
| Depositor: | This host strain was deposited by Prof. Dr B. Wren1. It was constructed by Dr E. Kay1. 1 Department of Infection Biology, London School of Hygiene and Tropical Medicine, London, UK |
| Containment level: | This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms, and its updates (see also the Belgian risk group classification). |
| Other culture collection numbers: | - |
| Medium: | LB-Lennox |
| Selection marker: | / |
| Temperature: | 37°C |
| Genotype: | F- λ- IN(rrnD-rrnE)1 rph-1 ΔlpxM ΔwecA-wzzE(gne) ΔwaaL ΔwzzB(wzD-wzE) (Source: Kay et al. (2022)) |
| Phenotype: | - |
| Properties: | This host strain is a derivative of the Hobby strain and can be used for bioengineering where purified products with reduced endotoxicity are desirable. Enterobacterial common antigen is no longer produced and a UDP N-acetylglucose epimerase has been chromosomally integrated increasing the availability of UDP-GalNAc. The O-antigen ligase has also been removed so that recombinant glycans are not removed from Und-P and attached to surface lipid. wzzB, the O-antigen chain length regulator of Escherichia coli, was removed and replaced by wzD-wzE, the chain length regulators from Streptococcus pneumoniae TIGR4, in the Hobby strain using CRISPR technology, in order to increase polymerization of plasmid expressed S. pneumoniae capsule glycans. |
| Further information: | This strain is part of a versatile suite of glycoengineering strains for various downstream applications, such as glycoconjugate vaccine production. |
| Original reference: | Kay et al., Microb. Cell Fact. 21 (2022), 66 [PMID: 35449016] [DOI: 10.1186/s12934-022-01792-7] |
| Restricted use: | - BCCM MTA - The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC. |
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Refer in your Materials and Methods: |
Escherichia coli K12 W3110 Sparrowhawk (LMBP 13419) is available at BCCM/GeneCorner. It was deposited by Prof. Dr B. Wren and was published in Kay et al., 2022. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.